规格
|
Excitation/Emission:
|
496⁄524, 497⁄592 |
Flow Cytometer Laser Lines:
|
488 |
Form:
|
Solid |
Product Line:
|
CellTrace™, Oregon Green® |
Reagent Type:
|
Cell Tracker Compounds, Cell Labeling Reagents |
Shipping Condition:
|
Room Temperature |
Solubility:
|
DMSO (Dimethylsulfoxide) |
Detection Method:
|
Fluorescent |
For Use With (Equipment):
|
Fluorescence Microscope, Flow Cytometer |
Label Type:
|
Classic Dyes |
Label or Dye:
|
Oregon Green® 488 |
储存
|
Contains 20 vials of Oregon Green® 488 carboxylic acid diacetate, SE (50 µg/vial).
Store in freezer (-5 to -30°C) and protect from light. |
Like CFDA SE, CellTrace™ Oregon Green® 488 (carboxy-DFFDA SE) should be a useful tool for following proliferating cells. This Oregon Green® 488 probe passively diffuses into cells, where it is colorless and nonfluorescent until its acetate groups are removed by intracellular esterases to yield a highly fluorescent, amine-reactive dye. Upon reaction with intracellular amines, the probe forms Oregon Green® 488 conjugates that are well-retained by cells. Unlike fluorescein conjugates, Oregon Green® 488 conjugates exhibit bright, photostable green fluorescence that is independent of pH at typical cellular pH values (pH-6-8).